The quadruple cortisol profile provides the most complete picture of cortisol rhythm throughout the day by tracking levels at four key times – morning peak (7:00-9:00 a.m.), midday level (~12:00 p.m.), afternoon decline (~4:00 p.m.), and nighttime level (~10:00 p.m.). It is the best method for diagnosing adrenal disorders, including Cushing’s syndrome, Addison’s disease, and chronic fatigue. It is used in cases of suspected metabolic disorders, hormonal imbalance, and chronic stress, providing information on whether the body is properly regulating cortisol secretion during the day and night.

The test requires a special tube (Salivette), which the patient can obtain from the laboratory.